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脑脊液的microRNA分析采用实时定量PCR技术
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JoVE Journal Medicina
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
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脑脊液的microRNA分析采用实时定量PCR技术

DOI:
10.3791/51172-v

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09:26 min

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January 22, 2014

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, , ,
1Medical School and Stanley S. Scott Cancer Center,LSU Health Sciences Center, 2Department of Biomedical, Surgery and Dental Sciences,University of Milan

Capítulos

  • 00:05Título
  • 01:24Reverse Transcription
  • 03:22Real-time PCR Amplification
  • 05:20Real-time PCR Data Analysis
  • 06:24Inter-plate Data Preparation and Analysis
  • 08:18Results: miRNA Expression Profiles from Cerebrospinal Fluid
  • 09:05Conclusion

Summary

Tadução automática

Tadução automática

我们描述了实时PCR的协议来分析小分子RNA在脑脊液（CSF）。除的RNA提取的协议，该过程可以扩展到从其它体液，细胞培养，或组织标本中提取的RNA。

Tags

Cerebrospinal FluidMicroRNAMiRNAQPCRGene RegulationBiomarkerProfilingExiqonGenEx Professional 5

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