This content is Free Access.
Capítulos
- 00:05Título
- 01:11Overview of Isolating DNA from Polytene Chromosomes by Laser Capture Microdissection
- 02:07Probe Preparation via Whole Genome Amplification
- 03:593D FISH on Whole-mount Ovarian Tissue
- 07:33WGA Kit Yields and FISH Labeling
- 09:31Conclusion
Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).
