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Biologia
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Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter
JoVE Journal
Biologia
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JoVE Journal Biologia
Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

DOI:
10.3791/51178-v

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07:37 min

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February 13, 2014

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, , ,
1Microbial Biotechnology, Institute Biology Leiden,Leiden University, 2Microbiology, Kluyver Centre for Genomics of Industrial Fermentation,Utrecht University

Capítulos

  • 00:05Título
  • 00:59COPAS Analysis
  • 03:35Pellet Sorting
  • 05:06Results: Measurement and Sorting of Streptomyces Pellets by COPAS
  • 07:13Conclusion

Summary

Tadução automática

Tadução automática

Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.

Tags

Keywords: StreptomycesCell PelletsComplex Object Parametric Analyzer And Sorter (COPAS)Mycelial PelletsPellet SizeCell FactoryBioreactorFilamentous BacteriaEnzyme ProductionAntibiotic ProductionHeterogeneous GrowthProductivity

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