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1Department of Biomedical and Molecular Sciences,Queen’s University, 2National Institute of Neurological Disorders and Stroke,Porter Neuroscience Research Center, 3Research Institute of Genome-Based Biofactory,National Institute of Advanced Industrial Science and Technology, 4The Robert H. Smith Faculty of Agriculture, Food and Environment, Institute of Biochemistry, Food Science, and Nutrition,The Hebrew University of Jerusalem
Capítulos
- 00:05Título
- 01:14Growing Single Ice Crystals
- 03:18Determining Singularity and Orientation o the Ice Crystal
- 04:37Absorption of Fluorescently Labeled Antifreeze Proteins to a Single Ice Crystal
- 07:20Results: Fluorescence-based Ice Plane Affinity Analysis Images
- 08:18Conclusion
Antivrieseiwitten (ACP's) binden zich aan specifieke ijsvlakken om ijsgroei te voorkomen of te vertragen. Fluorescentie-gebaseerde ijsvlakaffiniteitsanalyse (FIPA) is een wijziging van de oorspronkelijke ijsetsmethode voor de bepaling van AFP-gebonden ijsvlakken. AFP's zijn fluorescerend gelabeld, verwerkt in macroscopische enkelvoudige ijskristallen en gevisualiseerd onder UV-licht.
