Journal
/
Imunologia e Infecção
/
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer’s Patches Using Adoptive Transfer of Hematopoietic Progenitors

DOI:
10.3791/51189-v

•

13:34 min

•

March 17, 2014

•

,
1Department of Immunology,The University of Texas MD Anderson Cancer Center, 2The University of Texas Graduate School of Biomedical Sciences

Capítulos

  • 00:05Título
  • 01:56Hydrodynamic Gene Transfer
  • 02:54Adoptive Transfer of Common Dendritic Progenitors (CDPs)
  • 06:38Isolation and Enumeration of Peyer’s Patch Plasmacytoid Dendritic Cells
  • 09:36Results: Analysis of PP pDC in Ifnar/- Mice Upon Flt3L HGT and Adoptive Transfer of CPDs
  • 13:12Conclusion

Summary

Tadução automática

Tadução automática

This protocol describes experimental procedures to assess the differentiation of plasmacytoid dendritic cells in Peyer’s patch from common dendritic cell progenitors, using techniques involving FACS-mediated cell isolation, hydrodynamic gene transfer, and flow analysis of immune subsets in Peyer’s patch.

Tags

Keywords: Murine Plasmacytoid Dendritic CellsPeyer’s PatchesAdoptive TransferHematopoietic ProgenitorsCommon Dendritic Cell ProgenitorsFlt3 LigandIfnar-/- MiceFlow Cytometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/51189?article_type=v

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model
check_url/pt/51189?article_type=v

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer’s Patches
check_url/pt/51189?article_type=v

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells
check_url/pt/51189?article_type=v

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
check_url/pt/51189?article_type=v

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
check_url/pt/51189?article_type=v

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
check_url/pt/51189?article_type=v

Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
check_url/pt/51189?article_type=v

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy
check_url/pt/51189?article_type=v

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
check_url/pt/51189?article_type=v

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Read Article