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从肾脏双重否定αβ性T细胞的分离

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation of Double Negative αβ T Cells from the Kidney

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从肾脏双重否定αβ性T细胞的分离

DOI:

10.3791/51192-v

•

06:56 min

•

May 16, 2014

•

Maria N. Martina, Samatha Bandapalle, Hamid Rabb, Abdel R. Hamad

¹Department of Pathology,Johns Hopkins University School of Medicine, ²Department of Medicine,Johns Hopkins University School of Medicine

Capítulos

00:05Título
01:47Kidney Mononuclear Cell (KMNC) Isolation
03:48DN T Cell Isolation from CD45+ KMNC
05:26Results: Representative Kidney DN αβ T Cell Purity
06:31Conclusion

Summary

Tadução automática

DNabT细胞是罕见的外周T细胞之间;然而，他们是丰富的在某些非淋巴组织。从非淋巴组织中分离的DN T细胞的难度阻碍了其功能的分析尽管越来越多的认可的病理生理意义。我们描述了一种新的协议，用于从小鼠肾脏高度纯化的DN T细胞的隔离。

Tags

Double Negative T Cells DN T Cells Kidney Isolation Protocol CD45+ Selection Negative Selection CD4 CD8 MHC Class II CD1d Î±-galcer Tetramer Purity

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