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抗核抗体筛选使用Hep-2细胞
JoVE Journal
Biologia
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JoVE Journal Biologia
Anti-Nuclear Antibody Screening Using HEp-2 Cells
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抗核抗体筛选使用Hep-2细胞

DOI:
10.3791/51211-v

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13:01 min

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June 23, 2014

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, , ,
1IFA Development Manager,INOVA Diagnostics, Inc., 2IFA Development,INOVA Diagnostics, Inc., 3Product Manager, Rheumatology,INOVA Diagnostics, Inc., 4Medical Director,INOVA Diagnostics, Inc.

Capítulos

  • 00:05Título
  • 02:11Sample Preparation and Substrate Selection
  • 03:08Addition of Controls and Samples
  • 04:34Addition of Fluorescent Conjugate
  • 06:08Identification of Positive and Negative Results
  • 07:52Results: Cell Staining Patterns for HEp-2
  • 11:23Conclusion

Summary

Tadução automática

Tadução automática

间接免疫荧光（IIF）的测定法在传统上被用于抗核抗体（ANA）的人血清中的检测。这些抗体的存在可以在全身性自身免疫性风湿病（SARD）帮助诊断。该协议演示了如何有效地进行IIF技术，准确检测这些自身抗体。

Tags

Anti-nuclear AntibodyANAHEp-2 CellsIndirect Immunofluorescence (IIF)Automated Fluorescent MicroscopyNOVA ViewDigital Imaging SystemsSlide ProcessingFluorescent MicroscopyPattern RecognitionAutomated ReadingPatient Data IntegrityPatient Safety

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