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In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice
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Biologia
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JoVE Journal Biologia
In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

DOI:
10.3791/51229-v

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05:24 min

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March 22, 2014

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, ,
1Institute for Pharmacology,University of Heidelberg

Capítulos

  • 00:05Título
  • 01:20Preparation of siRNA/PEI Complex
  • 02:02Intrathecal Injection
  • 03:46Results: Intrathecal Injections and siRNA Downregulation of Protein
  • 05:00Conclusion

Summary

Tadução automática

Tadução automática

This report describes a simple and rapid technique of intrathecal needle puncture for a localized transfection of siRNA in the lumbar spinal cord in mouse under short lasting light anesthesia.

Tags

In VivoSiRNATransfectionGene KnockdownSpinal CordLumbar Intrathecal InjectionMiceNoninvasivePolyethyleniminePEISpinal FunctionsPharmacological AgentsRNA Modulators

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