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体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠

JoVE Journal
Biologia

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JoVE Journal Biologia

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

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体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠

DOI:

10.3791/51229-v

•

05:24 min

•

March 22, 2014

•

Christian Njoo, Celine Heinl, Rohini Kuner

¹Institute for Pharmacology,University of Heidelberg

Capítulos

00:05Título
01:20Preparation of siRNA/PEI Complex
02:02Intrathecal Injection
03:46Results: Intrathecal Injections and siRNA Downregulation of Protein
05:00Conclusion

Summary

Tadução automática

这份报告描述了鞘内穿刺针用于siRNA在小鼠脊髓腰段下短持久的浅麻醉局部转染一个简单而快速的方法。

Tags

In Vivo SiRNA Transfection Gene Knockdown Spinal Cord Lumbar Intrathecal Injection Mice Noninvasive Polyethylenimine PEI Spinal Functions Pharmacological Agents RNA Modulators

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