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使用吉布森大会和基因枪烟草中瞬时基因表达

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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

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使用吉布森大会和基因枪烟草中瞬时基因表达

DOI:

10.3791/51234-v

•

12:02 min

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April 18, 2014

•

Matthew D. Mattozzi², Mathias J. Voges^2,3, Pamela A. Silver², Jeffrey C. Way²

¹Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Department of Systems Biology,Harvard Medical School, ³Department of Biotechnology,Delft University of Technology

Capítulos

00:05Título
01:11Design of Sequences for Transient Expression
02:06Gibson Assembly of DNA Parts
06:16Biolistic Transfection of Tobacco with the Gene Gun
08:50Representative Images of Biolistically Transfected Tobacco Leaf Cells
11:18Conclusion

Summary

Tadução automática

这个工作描述了一种新颖的方法，用于选择性地靶向亚细胞器在植物中，使用BioRad公司基因枪进行测定。

Tags

Keywords: Alternative Splicing Chloroplast Cytosol Gibson Assembly GFP Gene Gun Metabolic Engineering Multi-organelle Targeting Peroxisome Synthetic Biology Tobacco Transient Gene Expression

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