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评估细胞死亡的吞噬细胞清除由Ligatable荧光探针试验行程

JoVE Journal
Medicina

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JoVE Journal Medicina

Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes

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评估细胞死亡的吞噬细胞清除由Ligatable荧光探针试验行程

DOI:

10.3791/51261-v

•

07:14 min

•

May 27, 2014

•

Candace L. Minchew², Vladimir V. Didenko²

¹Baylor College of Medicine, ²Michael E. DeBakey Veterans Affairs Medical Center

Capítulos

00:05Título
01:23Tyramide Signal Amplification (TSA) System
02:22Section Preparation
03:38VACC TOPO-based Staining
04:31TSA Signal Enhancement
06:12Results: Imaging Phagocytic Clearance
06:39Conclusion

Summary

Tadução automática

我们提出了选择性的一个新的荧光技术在主动吞噬细胞原位标记，它明确了在中风细胞的尸体。该方法对于评估脑反应缺血重要的，因为目前在缺血性脑吞噬细胞的只有一小部分参与细胞死亡的间隙。

Tags

Phagocytic Clearance Cell Death Experimental Stroke Fluorescent Probes Histochemical Approach Brain Ischemia Waste-management Phagocytes Cell Corpses Ischemic Injury DNA Breaks Topoisomerase Regenerative Processes

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