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激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

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激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量

DOI:

10.3791/51284-v

•

09:04 min

•

May 22, 2014

•

Melissa V. Fernandez, Elizabeth A. Miller, Nina Bhardwaj

¹Department of Pathology,New York University School of Medicine, ²Division of Infectious Diseases, Department of Medicine,Mount Sinai Medical Center, ³Division of Hematology and Oncology, Hess Center for Science and Medicine,Mount Sinai Medical Center

Capítulos

00:05Título
01:34Priming and Activating the Inflammasome
02:46Sample Collection
05:05Data Acquisition
06:52Results: Measuring TLR8 Priming with R848
08:33Conclusion

Summary

Tadução automática

树突状细胞（DC）分泌IL-1β响应于TLR8识别合成嘌呤，R848，接着NLRP3炎性体激活与尼日利亚菌素，因此，IL-1β可以用来测量NLRP3炎性活性。细胞内细胞因子染色法，免疫印迹和ELISA法用于准确地测量通过IL-1β表达NLRP3炎性引发和活化。

Tags

NLRP3 Inflammasome IL-1Î² Monocyte-derived Dendritic Cells R848 Nigericin Innate Immune Response Inflammation Caspase-1 TLR8 Bacterial Toxin Potassium Ionophore

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