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Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair
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Medicina
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JoVE Journal Medicina
Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

Sequential In vivo Imaging of Osteogenic Stem/Progenitor Cells During Fracture Repair

DOI:
10.3791/51289-v

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10:30 min

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May 23, 2014

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, , ,
1Center for Regenerative Medicine, Massachusetts General Hospital,Harvard Stem Cell Institute, 2Wellman Center for Photomedicine and Center for Systems Biology, Massachusetts General Hospital,Harvard Medical School

Capítulos

  • 00:05Título
  • 01:57Microfracture Injury
  • 04:35Intravital Imaging
  • 07:51Results: Representative Osteogenic Stem and Progenitor Cell Imaging
  • 09:54Conclusion

Summary

Tadução automática

Tadução automática

Quantitative measurement of bone progenitor function in fracture healing requires high resolution serial imaging technology. Here, protocols are provided for using intravital microscopy and osteo-lineage tracking to sequentially image and quantify the migration, proliferation and differentiation of endogenous osteogenic stem/progenitor cells in the process of repairing bone fracture.

Tags

Bone RepairOsteogenic Stem/progenitor CellsIn Vivo ImagingCell TracingMicrofractureCalvarial BoneBone RegenerationStem Cell Regulation

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