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Bioenergetics and the Oxidative Burst: Protocols for the Isolation and Evaluation of Human Leukocytes and Platelets
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Capítulos
- 00:05Título
- 02:33Cell Isolation and Plating
- 08:02Results: Representative Bioenergetic Index Analyses
- 10:11Conclusion
Blood leukocytes and platelets can be used as a marker of overall bioenergetic health of an individual and so have the potential to monitor pathological processes and the impact of treatments. Here we describe a method to isolate and measure mitochondrial function and the oxidative burst in these cells.
Tags
Keywords: BioenergeticsOxidative BurstLeukocytesPlateletsMitochondrial DysfunctionAtherosclerosisDiabetesSeptic ShockNeurodegenerative DiseasesHyperglycemiaInflammationMagnetic Bead IsolationCell AdhesionMicroplate RespirometryExtracellular Flux AnalyzerMonocytesLymphocytesNeutrophilsReactive Oxygen Species
