É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:26Maintenance of Microphage Cell Lines
- 02:04Programming the 96-well Shuttle System for Transfection
- 03:14Preparing the Reagents and Macrophages for Transfection
- 05:27Nucleofection of Macrophages with siRNA
- 07:45Recovery and Plating of Macrophages
- 09:35Stimulation of Macrophages with LPS and Monitoring the Induced Innate Immune Response, Gene Knockdown and Viability in Macrophages
- 10:58Results: Monitoring the Innate Immune Response
- 12:11Conclusion
