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DNA交联聚丙烯酰胺水凝胶的制备
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Biologia
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JoVE Journal Biologia
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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DNA交联聚丙烯酰胺水凝胶的制备

DOI:
10.3791/51323-v

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09:06 min

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August 27, 2014

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,
1New Jersey Neuroscience Institute,JFK Medical Center, 2Department of Biomedical Engineering,Rutgers University, 3Department of Mechanical and Aerospace Engineering,Rutgers University

Capítulos

  • 00:05Título
  • 01:46Preparation of DNA Gels
  • 05:34Gel Immobilization on Glass
  • 06:34Gel Functionalization
  • 07:34Results: Analysis of Stiffening and Softening DNA-crosslinked Polyacrylamide Hydrogels
  • 08:33Conclusion

Summary

Tadução automática

Tadução automática

我们的实验室已开发的DNA交联聚丙烯酰胺水凝胶，动态水凝胶体系，以更好地了解调节组织硬度对细胞功能的影响。这里，我们提供了示意图，说明和协议来制备这些凝胶。

Tags

Keywords: DNA-crosslinked Polyacrylamide HydrogelsMechanobiologyDynamic StiffnessDNA CrosslinksPolyacrylamide BackboneReversible ControlGel ElasticityFibroblastNeuron Behavior

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