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Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
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JoVE Journal Medicina
Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples

DOI:
10.3791/51332-v

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07:16 min

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March 14, 2014

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, , , , , , ,
1Department of Pathology,Icahn School of Medicine at Mount Sinai, 2Molecular Biology Program,Memorial Sloan-Kettering Cancer Center

Capítulos

  • 00:05Título
  • 01:17Harvesting and Processing of Human Prostrate Cancer Tissue
  • 02:18Generation of Cell Suspensions from Tissue Sections
  • 04:07Isolation of Prostrate Cancer Stem Cells (CSCs) by FACS
  • 05:56Results: Representative Human Prostrate Cancer Cell Populations
  • 06:50Conclusion

Summary

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The isolation of cancer stem cells (CSCs) directly from human tissues is requisite for their biological characterization. This manuscript describes a methodology for the isolation of prostate CSCs from human tissues, while also providing tips on troubleshooting difficult steps.

Tags

Cancer Stem CellsProstate CancerHLA Class IFlow CytometryHuman Tumor SamplesCell IsolationMolecular CharacterizationFunctional ValidationImmunodeficient Mice

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