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Capítulos
- 00:05Título
- 01:57pTIRF System Setup
- 05:54Chromaffin Cell Isolation
- 07:04pTIRF Image Acquisition
- 09:14Results: Monitoring Membrane Deformations with pTIRFM
- 11:58Conclusion
Polarization-based Total Internal Reflection Fluorescence Microscopy (pTIRFM) enables real-time detection of cell membrane dynamics. This article describes the implementation of pTIRFM for the study of membrane remodeling during regulated exocytosis. The technique is generalizable to other processes in cell biology that directly or indirectly involve changes in membrane shape.
