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共识脑源性蛋白，人类和小鼠脑蛋白质组使用两个2D-DIGE和Mini双向电泳免疫印迹法研究提取协议

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

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共识脑源性蛋白，人类和小鼠脑蛋白质组使用两个2D-DIGE和Mini双向电泳免疫印迹法研究提取协议

DOI:

10.3791/51339-v

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10:51 min

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April 10, 2014

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Capítulos

00:05Título
01:08Extracting Total Protein
03:07Quality Control and Dye Conjugation
05:28Fluorescence Difference Gel Electrophoresis (2D-DIGE)
07:36Mini-2DE for Quantitative Analysis
08:53Proteomics of Human and Mouse Tissue with Alzheimer Disease
10:26Conclusion

Summary

Tadução automática

用尿素/硫脲/ SDS缓冲液为人类和小鼠脑组织中一种常见的蛋白提取协议允许由蛋白质2D-DIGE并随后将其表征迷你2DE免疫印迹鉴定。这种方法使一个人从活组织切片检查和实验模型获得更多的可重复性和可靠的结果。

Tags

Brain Proteome 2D-DIGE Mini 2DE Immunoblotting Sample Preparation Human Post-mortem Protein Aggregates Amyloid-beta Alpha-synuclein Neurodegenerative Diseases Biomarkers Therapeutic Targets

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