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Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
JoVE Journal
Biologia
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JoVE Journal Biologia
Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

DOI:
10.3791/51342-v

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08:37 min

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May 05, 2014

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, , ,
1Institut de Biologie du Développement de Marseille,UMR7288 CNRS, Aix-Marseille Université

Capítulos

  • 00:05Título
  • 01:21Light Sheet and Detection Path Set Up
  • 03:24Mounting C. elegans Embryos
  • 05:28Recording C. elegans Development
  • 07:11Multi-channel Time-lapse Z-stack Imaging of C. elegans Embryonic Development
  • 08:10Conclusion

Summary

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This protocol describes the setup of a light sheet microscope and its implementation for in vivo imaging of C. elegans embryos.

Tags

Light Sheet MicroscopyIn Toto ImagingC. ElegansEmbryo DevelopmentLow Phototoxicity3D Time-lapseUpright MicroscopeOpto-mechanical SetupObservation Chamber

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