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Imunologia e Infecção
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Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

DOI:
10.3791/51345-v

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07:31 min

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March 28, 2014

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, , , , , ,
1Center for Micro-BioRobotics @SSSA,Istituto Italiano di Tecnologia, 2Department of Biology,University of Pisa, 3Center for Biomolecular Nanotechnologies @UniLe,Istituto Italiano di Tecnologia

Capítulos

  • 00:05Título
  • 00:56Nanoparticle Internalization in Blood Leukocytes and Purified Monocytes
  • 02:22Flow Cytometric Analysis
  • 03:44Results: Representative FITC-SiO2 Nanoparticle Internalization Analyses
  • 06:55Conclusion

Summary

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Tadução automática

Analysis of nanoparticle interaction with defined subpopulations of immune cells by flow cytometry.

Tags

Flow CytometryNanoparticle InteractionImmune CellsFITC-SiO2 NanoparticlesRhodamine-SiO2 NanoparticlesPrimary MicrogliaGFP-expressing Monocyte/macrophage LineageCell FluorescenceSide-scattered Light

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