在伴有海藻酸钠生产新基因的鉴定铜绿假单胞菌使用小型 himar1水手转座子介导的诱变

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

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在伴有海藻酸钠生产新基因的鉴定铜绿假单胞菌使用小型 himar1水手转座子介导的诱变

DOI:

10.3791/51346-v

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08:57 min

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March 10, 2014

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T. Ryan Withers, Yeshi Yin, Hongwei D. Yu

¹Department of Biochemistry and Microbiology, Joan C. Edwards School of Medicine,Marshall University

Capítulos

00:05Título
01:42Preparation of Bacterial Strains and Biparental Conjugation
04:42Detection and Isolation of Mucoid Colonies
06:00gDNA Restriction Digestion and Ligation and Inverse PCR and Sequence Analysis
07:07Results: Mini-himar1 Mariner Transposon-mediated Mutagenesis
08:28Conclusion

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这里我们描述了使用微型himar1水手转座子介导的诱变以产生高密度的插入突变体文库，以屏幕的协议，分离和鉴定中的原型的铜绿假单胞菌菌株PAO1新颖藻稳压器。

在伴有海藻酸钠生产新基因的鉴定<em>铜绿假单胞菌</em>使用小型<em> himar1</em>水手转座子介导的诱变

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