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A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents
JoVE Journal
Neurociência
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JoVE Journal Neurociência
A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents

A Method to Make a Craniotomy on the Ventral Skull of Neonate Rodents

DOI:
10.3791/51350-v

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08:30 min

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May 22, 2014

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, ,
1Department of Biology,The City University of New York, City College, 2Department of Biomedical Engineering,The City University of New York, City College

Capítulos

  • 00:05Título
  • 01:20Intubation
  • 03:24Performing the Craniotomy
  • 04:25Two-photo Imaging
  • 06:22Results: Electroporation of Neural Tracers and Microvessel Permeability
  • 08:09Conclusion

Summary

Tadução automática

Tadução automática

A surgical method is described to expose the ventral skull in neonate rats. Using this approach it is possible to open a craniotomy to perform acute electrophysiology and two-photon microscopy experiments in the brainstem of anesthetized pups.

Tags

Keywords: CraniotomyVentral SkullNeonate RodentsIn Vivo ExperimentsBrainBrainstemPonsElectrophysiologyImagingStructural ChangesElectrical ActivityBlood-brain BarrierAuditory BrainstemRatsMice

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