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Biologia
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Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy
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Biologia
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JoVE Journal Biologia
Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

Isolation and Culture of Adult Mouse Cardiomyocytes for Cell Signaling and in vitro Cardiac Hypertrophy

DOI:
10.3791/51357-v

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06:51 min

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May 21, 2014

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, , , ,
1Department of Biochemistry and Cancer Biology,University of Toledo College of Medicine and Life Sciences, 2Department of Physiology and Pharmacology,University of Toledo College of Medicine and Life Sciences

Capítulos

  • 00:05Título
  • 01:12Mouse Heart Removal and Cannulation
  • 02:54Heart Perfusion and Digestion
  • 03:25Cells Dissociation and Calcium Reintroduction
  • 05:10Cell Culture
  • 06:00Results: Successful Isolation Quantification
  • 06:29Conclusion

Summary

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Tadução automática

We describe a reliable method for isolation of adult mouse cardiomyocytes. This protocol yields a consistent result for the culture of functional adult cardiomyocytes from a variety of genetically modified mice.

Tags

Adult Mouse CardiomyocytesCell SignalingCardiac HypertrophyGenetically Modified MiceCardiomyocyte IsolationLangendorff PerfusionType II CollagenaseCardiovascular ResearchDrug Development

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