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小脑轴突的激光Nanosurgery<em>在体内</em

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Laser Nanosurgery of Cerebellar Axons In Vivo

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小脑轴突的激光Nanosurgery<em>在体内</em

DOI:

10.3791/51371-v

•

09:25 min

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July 28, 2014

•

Anna L. Allegra Mascaro, Leonardo Sacconi², Francesco Saverio Pavone^2,3,4

¹European Laboratory for Non-Linear Spectroscopy,University of Florence, ²National Institute of Optics,National Research Council, ³Department of Physics and Astronomy,University of Florence, ⁴International Center for Computational Neurophotonics (ICON Foundation)

Capítulos

00:05Título
02:02Axonal Labeling
04:42Optical Window on the Cerebellar Cortex
06:29In Vivo Multi-photon Laser Axotomy
07:56Results: Laser Nanosurgery of Cerebllar Axons In Vivo
08:44Conclusion

Summary

Tadução automática

双光子成像，其耦合到激光nanodissection，是有用的工具来研究退化和再生过程中对中枢神经系统具有亚细胞分辨率。该协议显示了如何标记，图像，并剖析单个登山纤维在体内小脑皮质。

Tags

Keywords: Laser Nanosurgery Cerebellar Axons In Vivo Two-photon Fluorescence Imaging Neuronal Regeneration Climbing Fibers Olivocerebellar Neurons Anterograde Tracing Dextran-conjugated Dye Cranial Window Ti:Sapphire Laser

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