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Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration

DOI:
10.3791/51397-v

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10:57 min

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March 18, 2014

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, ,
1UCL Institute of Ophthalmology,University College London, 2Department of Human Immunology,Centre for Cancer Biology, South Australia

Capítulos

  • 00:05Título
  • 01:26Coating of Culture Inserts
  • 02:08Dissection of Hindbrains from E11.5 Mouse Embryos
  • 05:40Hindbrain Explant Culture
  • 07:26Wholemount Immunofluorescent Staining of Hindbrain Explants
  • 09:00Results: Neuronal Migration in Mouse Hindbrain in Ex Vivo Culture
  • 10:28Conclusion

Summary

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Embryonic neurons are born in the ventricular zone of the neural tube, but migrate to reach appropriate targets. Facial branchiomotor (FBM) neurons are a useful model to study neuronal migration. This protocol describes the wholemount ex vivo culture of mouse embryo hindbrains to investigate mechanisms that regulate FBM migration.

Tags

Keywords: MouseHindbrainEx Vivo CultureFacial Branchiomotor Neuron MigrationEmbryonic NeuronsVentricular ZoneNeural Network FormationRhombomereSignaling PathwaysNeuronal MigrationCell CultureFunction-blocking AntibodiesSmall MoleculesHeparin BeadsRecombinant Proteins

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