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鼠标后脑<em>前体内</em>企业文化来研究面部Branchiomotor神经元迁移
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Mouse Hindbrain Ex Vivo Culture to Study Facial Branchiomotor Neuron Migration
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鼠标后脑<em>前体内</em>企业文化来研究面部Branchiomotor神经元迁移

DOI:
10.3791/51397-v

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10:57 min

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March 18, 2014

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, ,
1UCL Institute of Ophthalmology,University College London, 2Department of Human Immunology,Centre for Cancer Biology, South Australia

Capítulos

  • 00:05Título
  • 01:26Coating of Culture Inserts
  • 02:08Dissection of Hindbrains from E11.5 Mouse Embryos
  • 05:40Hindbrain Explant Culture
  • 07:26Wholemount Immunofluorescent Staining of Hindbrain Explants
  • 09:00Results: Neuronal Migration in Mouse Hindbrain in Ex Vivo Culture
  • 10:28Conclusion

Summary

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Tadução automática

胚胎神经元是出生在神经管的脑室区，但迁移到达到适当的目标。面部branchiomotor（FBM）的神经元，研究神​​经细胞迁移的有用模型。本协议描述的小鼠胚胎hindbrains平片的体外培养研究，规范FBM迁移机制。

Tags

Keywords: MouseHindbrainEx Vivo CultureFacial Branchiomotor Neuron MigrationEmbryonic NeuronsVentricular ZoneNeural Network FormationRhombomereSignaling PathwaysNeuronal MigrationCell CultureFunction-blocking AntibodiesSmall MoleculesHeparin BeadsRecombinant Proteins

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