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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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JoVE Journal Biologia
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

DOI:
10.3791/51406-v

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09:43 min

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April 23, 2014

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, ,
1Institute for Cell Engineering, Department of Neurology and Neuroscience,Johns Hopkins University School of Medicine

Capítulos

  • 00:05Título
  • 01:17Transduction
  • 03:40Co-culturing
  • 06:01Expanding the Induced Pluripotent Stem Cells
  • 07:10Results: Induced Pluripotent Stem Cell Characterization
  • 09:12Conclusion

Summary

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Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced efficiency.

Tags

Human Induced Pluripotent Stem CellsIPSCsSendai-virusIntegration-freeTransgene-freeReprogrammingSomatic CellsGenetic DiseasesCell TherapyDrug ScreeningDisease ModelingHigh EfficiencyCost-effective

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