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使用气相色谱怀孕期间分析脂肪酸组成的变化在大鼠肝组织

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Química

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JoVE Journal Química

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy

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使用气相色谱怀孕期间分析脂肪酸组成的变化在大鼠肝组织

DOI:

10.3791/51445-v

•

11:08 min

•

March 13, 2014

•

Helena L. Fisk, Annette L. West, Caroline E. Childs, Graham C. Burdge, Philip C. Calder

¹Human Development & Health Academic Unit, Faculty of Medicine,University of Southampton

Capítulos

00:05Título
01:28Preparation of a Total Lipid Extract
03:00Separation of Lipid Classes by Solid Phase Extraction
05:19Preparation of Fatty Acid Methyl Esters from Cholesteryl Esters
06:26Removal of Cholesterol Contamination and Gas Chromatographic Analysis
08:02Results: Identification and Quantification of Fatty Acids in Rat Liver Tissue by Gas Chromatography
10:24Conclusion

Summary

Tadução automática

妊娠导致显著改变母体组织中的脂肪酸组合物。血脂可通过气相色谱法来获得允许在怀孕期间大鼠中识别和个别类脂质的脂肪酸定量饲喂各种高，低脂肪饮食。

Tags

Gas Chromatography Fatty Acids Lipid Analysis Rat Liver Pregnancy Solid Phase Extraction Triacylglycerols Phospholipids Cholesteryl Esters Omega-3 Omega-6

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