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维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养
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Biologia
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JoVE Journal Biologia
Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
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维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养

DOI:
10.3791/51465-v

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07:13 min

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May 13, 2014

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, , , , , , , , ,
1Renal Research Laboratory,Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant,Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences,University of Trieste

Capítulos

  • 00:05Título
  • 01:46Isolation of Primary Osteoblasts
  • 03:36All-trans Retinoic Acid Protocol
  • 05:05Results: Osteocytes Generation using Retinoic Acid
  • 06:31Conclusion

Summary

Tadução automática

Tadução automática

原代小鼠成骨细胞与维甲酸治疗产生分枝细胞轴承骨细胞的形态学和分子特征的同质人口。该方法克服了获得和保持主骨细胞在培养的难度，并且可以是有利的，研究从转基因模型中的细胞。

Tags

Osteocyte CulturesPrimary Mouse OsteoblastsRetinoic AcidOsteocyte DifferentiationOsteoblast MarkersOsteocyte-specific MoleculesTransgenic ModelsExtracellular Matrix

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