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A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
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Capítulos
- 00:05Título
- 01:13Generating an LPS Aerosol
- 03:14Bronchoalveolar Lavage (BAG)
- 05:12Formalin Fixation of Lung Tissue for Histological Assessment
- 06:26Results: Representative Pulmonary Neutrophilia Data
- 08:04Conclusion
We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.
