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在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞
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JoVE Journal Medicina
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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在结直肠癌细胞的三维模型的入侵肿瘤微环境的分子剖析和<em>体外</em>成纤维细胞

DOI:
10.3791/51475-v

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10:33 min

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April 29, 2014

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, , , , , , , ,
1Cancer Sciences Unit,University of Southampton School of Medicine, 2University Surgical Unit,University of Southampton School of Medicine, 3Bioinformatics Unit,London Research Institute, Cancer Research UK

Capítulos

  • 00:05Título
  • 01:32Establishment of Primary Fibroblast Cultures from Colonic Explants
  • 03:18Organotypic Preparation
  • 06:52Organotypic Fixation and Laser Microdissection of the Invasive Margin
  • 08:47Results: MicroRNA Expression Profiling of Tissue from Laser-microdissected Organotypic CRC Models
  • 10:06Conclusion

Summary

Tadução automática

Tadução automática

激光显微切割细胞和基质由合成的，组织工程肠癌模型的分子分析代表了一种新的和可操作的方法在肿瘤细胞之间的界面来表征和解剖的独特生物学在浸润前沿和癌症相关的基质细胞。

Tags

Molecular ProfilingInvasive Tumor Microenvironment3D Colorectal Cancer ModelEx Vivo FibroblastsTumor InvasionMetastatic CascadeCancer-Associated StromaOrganotypic Co-CultureLaser MicrodissectionGenomic/Transcriptomic/Epigenetic ProfilingBiomarkersDrug Development

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