/
/
An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:19Anterograde Labeling of RGC Presynaptic Inputs
- 03:26Prepare Tissue for Imaging
- 05:56Results: Strategy to Assess Microglia-mediated Engulfment of RGC Presynaptic Inputs
- 07:09Conclusion
Microglia are the resident immune cells of the central nervous system (CNS) with a high capacity to phagocytose or engulf material in their extracellular environment. Here, a broadly applicable, reliable, and highly quantitative assay for visualizing and measuring microglia-mediated engulfment of synaptic components is described.
