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热生物催化：等温滴定量热法表征酶促反应

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Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

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热生物催化：等温滴定量热法表征酶促反应

DOI:

10.3791/51487-v

•

13:00 min

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April 04, 2014

•

Luca Mazzei, Stefano Ciurli, Barbara Zambelli

¹Laboratory of Bioinorganic Chemistry, Department of Pharmacy and Biotechnology,University of Bologna

Capítulos

00:05Título
02:01Preparing Samples and Performing the Experiment
06:47Data Analysis
09:24Results: Quantification of Enzyme Catalysis by Isothermal Titration Calorimetry
12:29Conclusion

Summary

Tadução automática

等温滴定量热法测量热流释放或吸收的化学反应。该方法可用于定量酶催化。在本文中，该协议有助于建立，运行试验和数据分析通常被描述，并应用于酶水解尿素由刀豆脲酶的特性。

Tags

Keywords: Isothermal Titration Calorimetry ITC Enzymatic Reactions Enzyme Kinetics Thermodynamics Kcat KM Enthalpy Urease Jack Bean

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