An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in <em>Arabidopsis</em>

Wenjing She; Daniel Grimanelli; C&#233;lia Baroux

doi:10.3791/51530

Journal

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Biologia

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定量，单细胞在全安装胚珠在染色质修饰和核体系结构分析的有效方法<em>拟南芥</em

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Biologia

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JoVE Journal Biologia

An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis

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定量，单细胞在全安装胚珠在染色质修饰和核体系结构分析的有效方法<em>拟南芥</em

DOI:

10.3791/51530-v

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09:33 min

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June 19, 2014

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Wenjing She, Daniel Grimanelli, Célia Baroux

¹Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich, ²Institut de Recherche pour le Développement (UMR 232), Centre National de la Recherche Scientifique (ERL 5300),Université de Montpellier II

Capítulos

00:05Título
01:15Tissue Fixation, Dissection and Embedding
03:21Tissue Processing
05:41Immunostaining and Imaging
07:32High-Resolution 3D Quantitative Imaging in Ovules
08:40Conclusion

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我们在这里提供了一个高效，可靠的协议进行免疫染色，荧光原位杂交，DNA染色后在整个安装拟南芥胚珠定量，高分辨率成像。该方法已成功地用于分析染色质修饰和核架构。

定量，单细胞在全安装胚珠在染色质修饰和核体系结构分析的有效方法<em>拟南芥</em

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