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Biologia
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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Biologia
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JoVE Journal Biologia
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

DOI:
10.3791/51544-v

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12:20 min

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August 06, 2014

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, , , ,
1Department of Bioengineering,University of Pennsylvania, 2Integrated DNA Technologies, Inc.

Capítulos

  • 00:05Título
  • 01:16Preparation of RBMBs
  • 03:56Probe Delivery
  • 09:04Image Acquisition
  • 10:19Results: RBMBs Enable the Imaging of Individual RNA Transcripts in Living Cells
  • 11:49Conclusion

Summary

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Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.

Tags

Real-time ImagingSingle Engineered RNA TranscriptsLiving CellsRatiometric Bimolecular BeaconsGene ExpressionSpatial RegulationTemporal RegulationOligonucleotide-based Optical ProbeFluorescent SignalHybridizationPhotostableRed-shiftedHighly Emissive Organic DyesWide-field Fluorescent MicroscopeReal-time VisualizationMicroporation

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