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Aip1p动力由R256H突变的肌动蛋白蚀变
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Biologia
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JoVE Journal Biologia
Aip1p Dynamics Are Altered by the R256H Mutation in Actin
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Aip1p动力由R256H突变的肌动蛋白蚀变

DOI:
10.3791/51551-v

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08:57 min

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July 30, 2014

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, , ,
1Department of Pediatrics, Roy J. and Lucille A. Carver College of Medicine,University of Iowa, 2Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine,University of Iowa

Capítulos

  • 00:04Título
  • 01:42Transforming the Plasmid into Yeast Cells
  • 03:36Visualizing Aip1p-GFP Protein Movement Using Total Internal Reflection Fluorescence Microscopy
  • 06:57Results: Quantification of Fluorescently Tagged Aip1p Movement in Wild Type and Mutant Cells
  • 08:29Conclusion

Summary

Tadução automática

Tadução automática

致病突变的肌动蛋白可以改变细胞骨架的功能。细胞骨架动力学是通过利用全内荧光显微镜荧光标记蛋白成像量化。作为一个例子，细胞骨架蛋白，Aip1p，已经改变了定位和运动在细胞中表达突变的肌动蛋白同种型，R256H。

Tags

ActinCytoskeletal DynamicsTIRF MicroscopyR256H MutationAip1pActin DepolymerizationLive Cell ImagingCytoskeletal FunctionRegulatory InteractionsProtein Function

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