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Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
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Biologia
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JoVE Journal Biologia
Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

DOI:
10.3791/51552-v

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07:22 min

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June 30, 2014

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,
1Department of Physiology,The University of Tennessee Health Science Center, 2Department of Orthopedic Surgery and Biomedical Engineering,The University of Tennessee Health Science Center

Capítulos

  • 00:05Título
  • 01:07Isolating the Rat Lung With Attached Heart
  • 02:25Blood Perfusion of the Isolated Rat Lung
  • 03:40Microvascular Infusion of the Lung
  • 06:05Vascular Permeability after Bacterial Lipopolysaccharide Treatment
  • 06:58Conclusion

Summary

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The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.

Tags

Single Microvessel PermeabilityIsolated Blood-perfused Rat LungReal-time ImagingPulmonary MicrovesselsPermeability ChangesFluorophore InfusionBacterial LipopolysaccharideVenular And Capillary Microvessel SegmentsLung PermeabilityLung Microvascular Permeability

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