É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:05Vessel Dissection and Imaging
- 03:18Automated Analysis
- 04:36Graphical Output
- 05:28Results: Representative Results from LC_Pro Analysis of Basal and Stimulated Ca2+ Dynamics and Histograms of Parameter Distributions
- 06:23Conclusion
Hier ein neuer Bereich von Interesse Analyseprotokoll auf Basis von Sortier Best-Fit-Ellipsen zu Regionen positives Signal innerhalb von zwei-dimensionalen Zeitbild Zeitraffer-Sequenzen zugeordnet wird demonstriert. Dieser Algorithmus kann Forschern ermöglichen, umfassend zu analysieren physiologischen Ca 2 +-Signale mit minimalen Benutzereingaben und Bias.
Tags
Based On The Given TextThe Key Keywords Are:
Automated AnalysisDynamic Ca2+ SignalsImage SequencesIntracellular Ca2+ SignalsFluorescent Ca2+ Indicator DyesMicroscopy TechniquesRegion Of Interest (ROI) DetectionOne-dimensional Line ScanTwo-dimensional Image SequencesEllipsesNoise FilteringCa2+ Signal ParametersAmplitudeDurationSpatial SpreadImageJRStatistical Analysis
