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高产净化<em>恶性疟原虫</em>裂殖子适用于Opsonizing抗体检测
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
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高产净化<em>恶性疟原虫</em>裂殖子适用于Opsonizing抗体检测

DOI:
10.3791/51590-v

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10:38 min

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July 17, 2014

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, ,
1Division of Infection and Immunity,Walter and Eliza Hall Institute of Medical Research, 2Department of Medical Biology,University of Melbourne

Capítulos

  • 00:05Título
  • 01:34Isolation of P. falciparum Merozoites
  • 04:42Quantifying Merozoite Concentration by Flow Cytometry
  • 06:03Phagocytosis Assay
  • 07:48Results: The Merzoite:THP-1 Ratio Influences the Degree of Phagocytosis Observed
  • 09:38Conclusion

Summary

Tadução automática

Tadução automática

测量抗体功能的关键是了解免疫力恶性疟原虫疟疾。这个方法描述了可行的裂殖子的纯化和调理作用的依赖性吞噬作用通过流式细胞术测量。

Tags

Keywords: Plasmodium FalciparumMerozoitesMalaria VaccineOpsonizing AntibodiesPhagocytosisTHP-1 CellsE64 Protease InhibitorHigh-yield PurificationAssay Development

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