Journal

Biologia

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

JoVE Journal
Biologia

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Biologia

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo

DOI:

10.3791/51593-v

•

11:44 min

•

September 18, 2014

•

Chih Hung Ku, Brian J. Ferguson

¹Department of Pathology,University of Cambridge

Capítulos

00:05Título
01:17Concatemerization of Immuno-stimulatory DNA Sequences
02:43DNA Purification and Analysis
05:55Transfection of dsDNA Concatemers In Vitro
06:47Transfection of dsDNA Concatemers In Vivo
08:29Processing of DNA Stimulated Samples and Quantitative PCR Analysis
10:32Results: Concatermerized DNA Stimulate an Innate Immune Response In Vitro and In Vivo
11:14Conclusion

Summary

Tadução automática

The aim of the protocol is to use optimal methods for stimulating the cytoplasmic DNA sensing pathways in cells and in vivo. This is achieved by improving the generation of long, double-stranded DNA during blunt-end ligation. Cells or mice are then transfected using a lipid transfection reagent.

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

In vitro and in vivo Bioluminescence Reporter Gene Imaging of Human Embryonic Stem Cells

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo