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反向遗传吗啉使用方法心脏心室射出转染多难靶组织中的斑马鱼幼虫
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Biologia
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JoVE Journal Biologia
Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva
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反向遗传吗啉使用方法心脏心室射出转染多难靶组织中的斑马鱼幼虫

DOI:
10.3791/51595-v

•

08:22 min

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June 17, 2014

•

,
1DFG-Center for Regenerative Therapies Dresden,Technische Universität Dresden

Capítulos

  • 00:05Título
  • 01:20Preparation of Glass Needles and Morpholino Solution
  • 02:26Larval Injections and Analysis
  • 05:14Assessment of Regenerative Outgrowth
  • 06:21Results: Transfection Using Cardiac Ventricular Injection
  • 07:52Conclusion

Summary

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Tadução automática

一个适应性强的反向遗传学方法，斑马鱼在后来的发展和生理平衡，如使用基因特异性吗啉脑室注射组织再生的阶段来评估基因的功能。

Tags

ZebrafishReverse GeneticsMorpholinoCardiac Ventricular InjectionGene FunctionEmbryogenesisFinfold RegenerationVertebrate Regeneration

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