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Omvendt Genetisk Morpholino tilgang med kardiel ventrikulær Injection til transfektion flere vanskelige at target væv i zebrafisk Larva
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Biologia
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JoVE Journal Biologia
Reverse Genetic Morpholino Approach Using Cardiac Ventricular Injection to Transfect Multiple Difficult-to-target Tissues in the Zebrafish Larva

Omvendt Genetisk Morpholino tilgang med kardiel ventrikulær Injection til transfektion flere vanskelige at target væv i zebrafisk Larva

DOI:
10.3791/51595-v

•

08:22 min

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June 17, 2014

•

,
1DFG-Center for Regenerative Therapies Dresden,Technische Universität Dresden

Capítulos

  • 00:05Título
  • 01:20Preparation of Glass Needles and Morpholino Solution
  • 02:26Larval Injections and Analysis
  • 05:14Assessment of Regenerative Outgrowth
  • 06:21Results: Transfection Using Cardiac Ventricular Injection
  • 07:52Conclusion

Summary

Tadução automática

Tadução automática

En tilpasningsdygtig reverse genetisk metode til zebrafisk til at vurdere gen funktion under senere stadier af udvikling og fysiologiske homeostase såsom væv regenerering hjælp intraventrikulære injektioner af gen-specifikke morpholinos.

Tags

ZebrafishReverse GeneticsMorpholinoCardiac Ventricular InjectionGene FunctionEmbryogenesisFinfold RegenerationVertebrate Regeneration

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