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The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
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Capítulos
- 00:05Título
- 01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
- 04:54FTA Preparation: Peptide Pulsing and Cell Washing
- 07:50FTA Preparation: CPD Cell Labeling
- 09:43FTA Assay in Response to VV/HIV Epitopes
- 11:45Conclusion
The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.
