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Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

DOI:
10.3791/51647-v

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10:04 min

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August 19, 2014

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, ,
1Department of Genetics and Biochemistry,Clemson University Eukaryotic Pathogens Innovation Center

Capítulos

  • 00:05Título
  • 01:12Transfection of PCF Parasites with the Fluorescent Reporter Construct
  • 03:28Making Stabilates
  • 04:24Thawing Frozen Stocks
  • 05:25Cytometer Setup
  • 06:17Counting Cells and Measure Fluorescence Using the Flow Cytometer
  • 07:33Data Analysis
  • 08:06Results: Monitoring Real-time Glycosome Dynamics in Live Parasites
  • 09:20Conclusion

Summary

Tadução automática

Tadução automática

Glycosome dynamics in African trypanosomes are difficult to study by traditional cell biology techniques such as electron and fluorescence microscopy. As a means of observing dynamic organelle behavior, a fluorescent-organelle reporter system has been used in conjunction with flow cytometry to monitor real-time glycosome dynamics in live parasites.

Tags

Keywords: Fluorescent ProteinsGlycosome DynamicsTrypanosoma BruceiHuman African TrypanosomiasisNaganaPeroxisomesGlycolysisFluorescence MicroscopyPTS2-eYFPFlow CytometryOrganelle Composition

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