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Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy
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Biologia
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JoVE Journal Biologia
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy

Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction Using Confocal Spinning Disk Microscopy

DOI:
10.3791/51666-v

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13:36 min

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June 24, 2014

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, , , , ,
1Max-Planck-Institute for Molecular Biomedicine and Institute of Cell Biology, 2Department of Internal Medicine,Yale Cardiovascular Research Center and Section of Cardiovascular Medicine

Capítulos

  • 00:05Título
  • 02:10Isolation of Rat Neonatal Cardiomyocytes
  • 06:52Lentiviral Transduction
  • 07:55Adenoviral Transduction
  • 08:55Live Cell Imaging
  • 11:22Results: Confocal Spinning Disk Microscopy Enables Functional Studies of Proteins in Rat Neonatal Cardiomyocytes
  • 13:01Conclusion

Summary

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This protocol describes a method of live cell imaging using primary rat neonatal cardiomyocytes following lentiviral and adenoviral transduction using confocal spinning disk microscopy. This enables detailed observations of cellular processes in living cardiomyocytes.

Tags

Keywords: Live Cell ImagingPrimary Rat Neonatal CardiomyocytesAdenoviral TransductionLentiviral TransductionConfocal Spinning Disk MicroscopyCardiovascular ResearchCellular EventsPhototoxicityTimelapse ImagingGene ExpressionProtein FunctionSiRNAChemical Modulators

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