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冷冻断裂/冷冻蚀刻的生物电子显微技术的基本要素
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Biologia
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JoVE Journal Biologia
Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy
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冷冻断裂/冷冻蚀刻的生物电子显微技术的基本要素

DOI:
10.3791/51694-v

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11:17 min

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September 11, 2014

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1Department of Pediatrics, Center for Environmental Medicine, Asthma, and Lung Biology,The University of North Carolina at Chapel Hill

Capítulos

  • 00:05Título
  • 02:15Freezing and Storage of Specimens in Advance of Freeze-fracture
  • 04:43Operation of the Freeze-fracture Instrument and Specimen Processing
  • 08:45Results: Structures Revealed by Freeze-fracture
  • 10:28Conclusion

Summary

Tadução automática

Tadução automática

基本的技术和用于检查通过透射电子显微镜生物标本和纳米材料的冷冻断裂加工的精炼进行说明。这种技术对于揭示超微结构特点和生物膜的专业和获得超微结构水平尺寸和空间数据在材料科学和纳米技术产品的首选方法。

Tags

Freeze-fractureFreeze-etchElectron MicroscopyBiological SpecimensCell MembranesUltrarapid FreezingCryoprotective AgentsFracturingCarbon-platinum ReplicationThree-dimensional DetailCell UltrastructureCell BiologyNanotechnologyMaterials Science

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