Journal
/
Biologia
/
DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

DOI:
10.3791/51715-v

•

12:24 min

•

July 21, 2014

•

, ,
1Physical Biosciences Division,Lawrence Berkeley National Laboratory

Capítulos

  • 00:05Título
  • 01:57Determine Gene Target for Response Regulator Using Electrophoretic Mobility Shift Assay
  • 03:54Verify Target Enrichment after Genomic DNA-protein Binding
  • 07:19DNA Labeling and Array Hybridization
  • 10:11Results: DAP-chip Analysis of Gene Targets and Binding Sites for Response Regulators
  • 11:51Conclusion

Summary

Tadução automática

Tadução automática

This video article describes an in vitro microarray based method to determine the gene targets and binding sites for two component system response regulators.

Tags

Keywords: DNA-affinity-purified ChipDAP-chipBacterial Two Component Regulatory SystemsGene TargetsTranscription FactorsChIP-chipResponse RegulatorsIn Vitro MicroarrayAffinity PurificationTiling ArrayBinding Site MotifsDesulfovibrio Vulgaris HildenboroughSigma54-dependent Response Regulator

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/51715?article_type=v

Bacterial Gene Expression Analysis Using Microarrays
check_url/pt/51715?article_type=v

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
check_url/pt/51715?article_type=v

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
check_url/pt/51715?article_type=v

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
check_url/pt/51715?article_type=v

Determining the Contribution of the Energy Systems During Exercise
check_url/pt/51715?article_type=v

The Use of Ex Vivo Whole-organ Imaging and Quantitative Tissue Histology to Determine the Bio-distribution of Fluorescently Labeled Molecules
check_url/pt/51715?article_type=v

Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
check_url/pt/51715?article_type=v

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
check_url/pt/51715?article_type=v

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
check_url/pt/51715?article_type=v

Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation

Read Article