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In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
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Biologia
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JoVE Journal Biologia
In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

DOI:
10.3791/51725-v

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08:35 min

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July 19, 2014

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, , ,
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences,Swiss Institute for Experimental Cancer Research, 2DanStem,University of Copenhagen

Capítulos

  • 00:05Título
  • 01:55Dissection of Dorsal Pancreatic Bud from Mouse Embryos
  • 04:19Plating and Culturing of Dispersed Cells
  • 06:06Results: In Vitro Expansion and Differentiation of Embryonic Pancreatic Progenitors
  • 07:20Conclusion

Summary

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Tadução automática

The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.

Tags

Keywords: In Vitro Pancreas OrganogenesisMouse Embryonic Progenitors3D CulturePancreatic ProgenitorsPancreas DevelopmentPancreatic OrganoidsPancreatic MorphogenesisPancreatic Cell DifferentiationEpithelial PolarizationBranching MorphogenesisMechanical CuesCell Tensegrity

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