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In Vitro Pancreas Organogenesis fra spredte Mouse Embryonale Progenitorer
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Biologia
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JoVE Journal Biologia
In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

In Vitro Pancreas Organogenesis fra spredte Mouse Embryonale Progenitorer

DOI:
10.3791/51725-v

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08:35 min

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July 19, 2014

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, , ,
1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences,Swiss Institute for Experimental Cancer Research, 2DanStem,University of Copenhagen

Capítulos

  • 00:05Título
  • 01:55Dissection of Dorsal Pancreatic Bud from Mouse Embryos
  • 04:19Plating and Culturing of Dispersed Cells
  • 06:06Results: In Vitro Expansion and Differentiation of Embryonic Pancreatic Progenitors
  • 07:20Conclusion

Summary

Tadução automática

Tadução automática

Den tredimensionale kultur beskrevet i denne protokol metode sammenfatter bugspytkirtlen udvikling fra spredte embryonale mus bugspytkirtel stamfædre, herunder deres betydelige ekspansion, differentiering og morfogenese i en forgrenet orgel. Denne metode er modtagelig for billedbehandling, funktionelle indblanding og manipulation af den niche.

Tags

In Vitro Pancreas OrganogenesisMouse Embryonic Progenitors3D CulturePancreatic ProgenitorsPancreas DevelopmentPancreatic OrganoidsPancreatic MorphogenesisPancreatic Cell DifferentiationEpithelial PolarizationBranching MorphogenesisMechanical CuesCell Tensegrity

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